Oligo Tm (Wallace)
Wallace rule for short DNA oligos.
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How to use
Tm
Rough short-oligo estimate only.
Educational use only. Biology models are simplified; clinical and field decisions need appropriate expertise and measurement.
Wallace rule for short DNA oligos.
Rough short-oligo estimate only.
Educational use only. Biology models are simplified; clinical and field decisions need appropriate expertise and measurement.
Model / formula
Tm ≈ 2(A+T) + 4(G+C) — short primers only
Result
Tm ≈ 28.0000 °C (Wallace rule)